Associations between GHR and IGF-1 Gene Polymorphisms, and Reproductive Traits in Wenchang Chickens

Authors : Hui-fang LI, Wen-qi ZHU, Kuan-wei CHEN, Xu WU, Qing-ping TANG, Yu-shi GAO

Pages : 281-285

View : 10 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Alleles of physiological candidate genes for reproductive traits, insulin-like growth factor-1 insert ignore into journalissuearticles values(IGF-1);, and growth hormone receptor insert ignore into journalissuearticles values(GHR); were assessed to determine their associations with total egg production insert ignore into journalissuearticles values(NE);, average days of continual egg-laying insert ignore into journalissuearticles values(ADCE);, and number of double yolk eggs insert ignore into journalissuearticles values(DYE); in Wenchang chickens insert ignore into journalissuearticles values(Chinese indigenous breed);. PCR-RFLP was used for genotype identification. The frequency of restriction enzyme C1/C2 alleles in the population was 0.53 insert ignore into journalissuearticles values(C1); and 0.47 insert ignore into journalissuearticles values(C2); for IGF-1. For GHR-intron 2 it was 0.06 and 0.94 for insert ignore into journalissuearticles values(A1); and insert ignore into journalissuearticles values(A2);, respectively, while for GHR-intron 5 it was 0.20 insert ignore into journalissuearticles values(B1); and 0.80 insert ignore into journalissuearticles values(B2);. Four significant associations were found insert ignore into journalissuearticles values(P < 0.05);: between IGF-1 polymorphism, and NE insert ignore into journalissuearticles values(300 d);, NE insert ignore into journalissuearticles values(400 d);, and ADCE, and between GHR polymorphism and DYE. Two significant effects were observed: for IGF-1 and NE insert ignore into journalissuearticles values(300 d);, and for GHR intron 2 and DYE. The current research supports the effects of GHR and IGF-1 genes on the reproductive traits of chickens.
Keywords : Chicken, IGF 1, GHR, reproduction, single nucleotide polymorphism SNP,