Investigation of Chicken Infectious Anemia Virus Infection by PCR and ELISA in Chicken Flocks

Authors : H. Hüseyin HADİMLİ, Osman ERGANİŞ, Leyla GÜLER, U. Sait UÇAN

Pages : 79-84

View : 16 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Presence of the chicken infectious anemia virus insert ignore into journalissuearticles values(CIAV); infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks insert ignore into journalissuearticles values(16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers);, 922 serum samples were tested for CIAV-specific antibodies using a commercially available competitive ELISA. CIAV antibodies were positive in 609 insert ignore into journalissuearticles values(66.0%); sera from 34 flocks insert ignore into journalissuearticles values(89.5%);. A total of 95 thymus samples from 25 flocks, 57 samples from 15 commercial layers, and 38 samples from 10 commercial broiler flocks were tested by PCR. In 53 insert ignore into journalissuearticles values(55.8%); thymus samples from 20 flocks insert ignore into journalissuearticles values(80.0%); CIAV-specific DNA was detected. In commercial layers antibodies were detected in 15 insert ignore into journalissuearticles values(93.7%); of 16 flocks and viral DNA was detected in 13 insert ignore into journalissuearticles values(86.6%); of 15 flocks. In commercial broilers both CIAV antibodies and DNA were detected in 7 insert ignore into journalissuearticles values(70%); of 10 flocks tested. While seropositivity was detected in 12 insert ignore into journalissuearticles values(100%); of breeding broilers insert ignore into journalissuearticles values(8); and layer insert ignore into journalissuearticles values(4); flocks tested, no PCR was performed in these flocks. The study showed high prevalence of subclinical CIAV infection in the investigated chicken flocks.
Keywords : Chicken infectious anemia virus, ELISA, PCR, chicken