Harvesting and Evaluation of Riverine Buffalo Follicular Oocytes

Authors : Huma JAMIL, Hafız Abdus SAMAD, Zafar İqbal QURESHI, Najeeb Ur REHMAN

Pages : 25-30

View : 17 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The low superovulatory response and poor recovery rate of oocytes are major impediments to the application of in vitro fertilization and embryo transfer technology in buffaloes. The present study aimed to evaluate the comparative efficacy of oocyte collection methods, season insert ignore into journalissuearticles values(low and peak breeding season);, and ovarian status insert ignore into journalissuearticles values(presence or absence of corpus luteum); on the recovery rate and quality of recovered buffalo follicular oocytes. For this purpose riverine buffalo ovaries were collected from buffaloes slaughtered at Faisalabad Municipal Corporation Slaughter House. To study the efficiency of 3 different recovery methods the ovaries were dissected insert ignore into journalissuearticles values(n = 291);, punctured insert ignore into journalissuearticles values(n = 301);, or aspirated insert ignore into journalissuearticles values(n = 298);. In all, 675 oocytes were recovered by dissection, 441 by puncture, and 363 by aspiration. The results of the present study revealed that a significantly higher insert ignore into journalissuearticles values(P < 0.05); oocyte recovery rate was obtained from the ovaries collected during the peak-breeding season and from those in which the corpus luteum was absent. Of the 3 oocyte recovery methods used, dissection yielded the highest percentage insert ignore into journalissuearticles values(36.74%); of type I oocytes, followed by puncture insert ignore into journalissuearticles values(32.87%);, and aspiration insert ignore into journalissuearticles values(19.83%);.
Keywords : Buffalo, oocytes harvesting method, ovary, breeding season, corpus luteum