Coagulase Gene Polymorphism of Staphylococcus aureus Isolated from Subclinical Bovine Mastitis

Authors : Özkan ASLANTAŞ, Cemil DEMİR, Hülya TÜRÜTOĞLU, Zafer CANTEKİN

Pages : 253-257

View : 10 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities insert ignore into journalissuearticles values(Hatay, Gaziantep, and Burdur);. Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction insert ignore into journalissuearticles values(PCR); products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism insert ignore into journalissuearticles values(RFLP); of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.
Keywords : Staphylococcus aureus, RFLP PCR, mastitis