Effect of Astragalus and Astragalus Polysaccharide on Antimicrobial Peptides PR-39 and Protegrin-1 Gene Expression in Pigs

Authors : Youjin TU, Chunlan XU, Yizhen WANG, Guojia YAO, Tizhong SHAN

Pages : 325-330

View : 8 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This experiment was conducted to study the effects of micron Astragalus, 80 mesh Astragalus and Astragalus polysaccharide insert ignore into journalissuearticles values(APS); on antimicrobial peptides PR-39 and protegrin-1 gene expression in pigs using semi-quantitative RT-PCR analysis. One hundred twenty 60-day-old Duroc x Landrace x Yorkshire piglets insert ignore into journalissuearticles values(21.88 ± 1.26 kg of average initial body weight); were randomly allotted to 4 treatments. Each treatment had 3 replicates insert ignore into journalissuearticles values(i.e. pens); with 10 pigs per pen. The basal diets were supplemented with 0, 5 g kg-1 APS, 5 g kg-1 80 mesh Astragalus, and 5 g kg-1 micron Astragalus, respectively, and the feeding experiment lasted 30 days. After the feeding experiment, 12 pigs with 3 animals in each treatment were chosen to determine the effect of Astragalus or APS on PR-39 and protegrin-1 mRNA expression of pigs. The results showed that supplementation with 5 g kg-1 micron Astragalus significantly insert ignore into journalissuearticles values(P < 0.05); enhanced antimicrobial peptides PR-39 and protegrin-1 gene expression. Pigs fed the diet containing 5 g kg-1 80 mesh Astragalus also had higher PR-39 and protegrin-1 gene expression level than did the control insert ignore into journalissuearticles values(P < 0.05);. However, micron Astragalus was more effective than 80 mesh Astragalus in enhancing PR-39 and protegrin-1 gene expression. Supplementation with 5 g kg-1 APS reduced PR-39 and protegrin-1 gene expression insert ignore into journalissuearticles values(P < 0.05);.
Keywords : Astragalus, PR 39, protegrin 1, antimicrobial peptide, pigs