Characterization of Footrot Bacteria Dichelobacter nodosus Using PCR Amplification and DNA Sequence Analysis

Authors : İfakat Tülay ÇAĞATAY, Jon G. H. HICKFORD

Pages : 53-59

View : 14 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Dichelobacter nodosus is an essential causative agent of footrot in ruminants, particularly in sheep, goats and cattle. In this study, more than 100 footrot samples were collected from 4 different farming regions in New Zealand insert ignore into journalissuearticles values(NZ);. Selective media were chosen and isolation and routine growth conditions were optimized for NZ D. nodosus serotypes. Approximately 1000 primary plates were anaerobically subcultured several times and examined with Gram staining in order to detect single colonies of D. nodosus. Both the variable region and a part of the conserved region of fimbrial subunit gene insert ignore into journalissuearticles values(fimA); were amplified from bacterial DNA using polymerase chain reaction insert ignore into journalissuearticles values(PCR);. On the basis of PCR and DNA sequencing, 6 new D. nodosus isolates were identified and characterized from NZ. These sequences of D. nodosus fimA gene from NZ were genetically different from previously reported strains in the GenBank insert ignore into journalissuearticles values(NCBI); and commercial vaccine strains.
Keywords : Footrot, Dichelobacter nodosus, fimA gene, PCR, DNA sequencing