Determination of the Seroprevalence of Leptospirosis in Cattle by MAT and ELISA in Hatay, Turkey

Authors : Özkan ASLANTAŞ

Pages : 1019-1024

View : 12 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Five hundred and twelve serum samples collected from randomly selected cattle of different age and sex from the towns and central villages of Hatay during April-July 2003 were tested for antibodies against 3 different Leptospira interrogans serovars insert ignore into journalissuearticles values(grippotyphosa, hardjo and icterohaemorrhagie); using a microscopic agglutination test insert ignore into journalissuearticles values(MAT); and enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(ELISA);. Forty-five insert ignore into journalissuearticles values(8.8%); and 72 insert ignore into journalissuearticles values(14%); of the 512 serum samples tested were detected to be positive with MAT and ELISA, respectively. Of the 45 MAT positive serum samples, 26 insert ignore into journalissuearticles values(57.7%); were positive against serovar grippotyphosa, 9 insert ignore into journalissuearticles values(20%); were positive against serovar hardjo, 1 insert ignore into journalissuearticles values(0.2%); was positive against serovar icterohaemorrhagie and 9 insert ignore into journalissuearticles values(20%); were positive against serovar grippotyphosa + serovar hardjo. Of the 72 ELISA positive serum samples, 32 insert ignore into journalissuearticles values(44.4%); were positive against serovar grippotyphosa, 15 insert ignore into journalissuearticles values(20.8%); were positive against serovar hardjo, 2 insert ignore into journalissuearticles values(2.8%); were positive against serovar icterohaemorrhagie and 23 insert ignore into journalissuearticles values(31.9%); were positive against serovar grippotyphosa + serovar hardjo. The dominant serovar was grippotyphosa. Statistical analyses indicated a significant difference in seroprevalence among locations insert ignore into journalissuearticles values(P < 0.05);; however, no significant differences in positivity rates were found with respect to the age or sex of the animals insert ignore into journalissuearticles values(P > 0.05);.
Keywords : Leptospirosis, cattle, MAT, ELISA