Cloning of Nucleoprotein Gene (NP) of Rinderpest Virus (RPV) RBOK Vaccine Strain

Authors : Şükrü TONBAK, Aykut ÖZDARENDELİ, Yusuf BOLAT

Pages : 297-301

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In the present study cloning of the NP gene in a cloning vector is reported. For this purpose, Vero cells were infected with rinderpest RBOK vaccine strain. Total RNA was obtained from the infected cells and reverse-transcription insert ignore into journalissuearticles values(RT); was performed to get cDNAs of NP gene. Afterwards, the NP gene was amplified by polymerase chain reaction insert ignore into journalissuearticles values(PCR); with the primers specific to the NP gene. The NP gene, 1575 bp in length, was cloned into pGEM-3Zf insert ignore into journalissuearticles values(-); cloning vector. PCR screening and enzyme digestion assays carried out to verify the presence of the NP gene.
Keywords : Rinderpest virus, nucleoprotein gene, cloning