Isolation of Arcanobacterium (Actinomyces) pyogenes from Abscessed Cattle Kidney and Identification by PCR

Authors : Hasan Basri ERTAŞ, Ayşe KILIÇ, Gökben ÖZBEY, Adile MUZ

Pages : 455-459

View : 15 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle. A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 insert ignore into journalissuearticles values(40%); of the samples examined. DNA extracted from the isolates were amplified by Polymerase Chain Reaction insert ignore into journalissuearticles values(PCR); using specific primers derived from plo gene of A. pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.
Keywords : Arcanobacterium pyogenes, PCR, cattle, kidney