The Effect of Ascorbic Acid on the Freezability of Ram Semen Diluted with Extenders Containing Different Proportions of Glycerol

Authors : Mustafa SÖNMEZ, Eşref DEMİRCİ

Pages : 893-899

View : 11 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study was conducted to investigate the effects of ascorbic acid on the freezability and spermatological characteristics of ram semen diluted with Tris-glucose-egg yolk based extenders containing different proportions of glycerol. Six Akkaraman rams were used. Semen was collected by electroejaculator once every 3 days. The spermatological characteristics of the collected semen samples were determined. Pooled and diluted semen was cooled slowly to + 4 ºC over 2 h. Cooled semen was divided into 20 groups. Each of the 20 semen groups was diluted at a 1:1 ratio with one of the extender groups containing different proportions of glycerol insert ignore into journalissuearticles values(0%, 1%, 3%, 5%, and 7%); and ascorbic acid insert ignore into journalissuearticles values(0, 0.5, 1 and 2 mg/ml);. Diluted semen was packaged in 0.25 ml French straws and equilibrated for 4 h before freezing. The semen in straws was frozen in liquid nitrogen vapor. The semen was thawed for 25 s at +38 ºC. There was no significant difference in the spermatological characteristics depending on the increase in the proportions of acorbic acid after glycerolization-equilibration and freezing-thawing. While the increase in glycerol level in diluted semen reduced motility insert ignore into journalissuearticles values(P < 0.05);, it raised the dead spermatozoa rate, and damaged the acrosome rate and total abnormal spermatozoa rate insert ignore into journalissuearticles values(P < 0.05); after glycerolization and equilibration. However, the increase in the glycerol level in diluted semen raised motility, and damaged the acrosome rate and total abnormal spermatozoa rate insert ignore into journalissuearticles values(P < 0.05);, while it reduced the dead spermatozoa rate insert ignore into journalissuearticles values(P < 0.05); after freezing and thawing compared to the control diluent. In conclusion, the best results after freezing and thawing were observed in diluted semen groups containing 5% glycerol although the increase in the glycerol level insert ignore into journalissuearticles values(3%-7%); after glycerolization and equilibration in diluted semen had negative effects on spermatological characteristics. Moreover, the increase in the proportions of ascorbic acid in extended semen did not change any spermatological characteristics.
Keywords : Ram, semen, freezing, ascorbic acid, glycerol