The Effects of Various BSA Levels in Different Media on Development in In Vitro Culture of Mouse Embryos

Authors : Mithat EVECEN, Serhat PABUCÇUOĞLU, Serhat ALKAN, İ. Kamuran İLERİ

Pages : 337-342

View : 18 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Various BSA insert ignore into journalissuearticles values(bovine serum albumin); levels and media effects on development of 2-cell stage mouse embryos in in vitro culture were investigated in this 2 stage study. In the first stage, 1400 2-celled mouse embryos were cultured in 2 various BSA-bearing culture media for 96 h and directly observed microscopically. In the second stage, 1024 2-celled mouse embryos cultured for the same period and cell numbers insert ignore into journalissuearticles values(nuclei of cells); were determined by staining techniques. Embryos were cultured in both stages in M 16 and Whitten`s media containing 0 insert ignore into journalissuearticles values(control);, 0.3, 1, 3, 9, 18 and 36 mg/ml of BSA. The highest blastocyst rate was 94.57% ± 7.43, observed in 3 mg/ml BSA-containing Whitten`s medium group insert ignore into journalissuearticles values(P < 0.05);. The highest hatching + hatched blastocyst rates were obtained in the same group with 67.70% ± 25.22. The highest nucleus number in the M 16 medium was in the 1 mg/ml BSA group insert ignore into journalissuearticles values(95.45 ± 9.61);. In the M16 medium group, the 3 mg/ml BSA group showed the highest development rate in the first stage of the study; however, the nucleus numbers in this group insert ignore into journalissuearticles values(81.37 ± 18.31);, were significantly lower than the nucleus numbers of the 1 mg/ml BSA group insert ignore into journalissuearticles values(P < 0.05);. It was concluded that the optimal benefit to in vitro culture of 2-cell mouse embryos could be maintained when BSA was used as a protein source at 1 mg/ml and 3 mg/ml doses, and that M16 or Whitten`s media could be used successfully as a culture medium. Developmental evaluations of in vitro cultured mouse embryos could be confusing when only the native route was employed, and a staining technique could give more reliable results.
Keywords : Mouse, embryo, medium, BSA, protein