Immunohistochemical Distribution of Cells Containing Insulin, Glucagon and Somatostatin in the Goose (Anser anser) Pancreas

Authors : Nurhayat GÜLMEZ*, Hakan KOCAMIŞ, Şahin ASLAN, Mümtaz NAZLI

Pages : 403-407

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The purpose of the present study was to determine the distribution of cells containing glucagon insert ignore into journalissuearticles values(A);, insulin insert ignore into journalissuearticles values(B);, and somatostatin insert ignore into journalissuearticles values(D); in the goose pancreas. Small pieces of tissue from 5 adult geese insert ignore into journalissuearticles values(Anser anser); pancreases were dissected under deep ether anaesthesia. Sections were stained with Crossmon`s connective tissue stain for general observations and Gomori`s method for pancreatic islet cells. Sections were further processed for standard immunohistochemical techniques using the avidin-biotin-complex method for the distribution of A, B and D cells. There were no B cells inside the A islets, which were mainly composed of A cells. B islets were mainly composed of insulin-producing B cells arranged in a circular fashion around blood capillaries. Granules containing insulin were localised in blood vessel sites of the B cells. Although D cells were distributed in almost all sites of the A islets, they were mainly localised at the periphery of the B islets. D cells throughout the pancreas were more common than that of B and A cells. The distribution of cells containing insulin, glucagon and somatostatin in the goose pancreas, with small differences, was similar to that of other avian species.
Keywords : Goose pancreas, immunohistochemistry, insulin, glucagon, somatostatin