Detection of Ehrlichia canis in Dogs by IFA Test and Dot-ELISA

Authors : Jale ERDEĞER

Pages : 767-773

View : 10 | Download : 3

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Ehrlichia canis is the causative agent of canine monocytic ehrlichiosis insert ignore into journalissuearticles values(CME);. The aim of this study was to detect E. canis antibodies in dogs by the IFA and dot-ELISA techniques. Blood sera from 239 dogs were collected as material. IFA test and dot-ELISA were performed with commercially available test kits. The IFA test was applied according to the procedure in the IFA kit. The Immunocomb test system was used in dot-ELISA. Agreement between these techniques was analysed by the kappa insert ignore into journalissuearticles values(K); test. The specificity and sensitivity of dot-ELISA were calculated. Positive results were observed from 162 insert ignore into journalissuearticles values(67.8%); and 137 insert ignore into journalissuearticles values(53.3%); of 239 dogs by IFA test and dot-ELISA, respectively. Among 91 dogs with clinical signs of CME, 56 insert ignore into journalissuearticles values(61.5%); and 46 insert ignore into journalissuearticles values(50.5%);; of 31 apparently healthy dogs, 8 insert ignore into journalissuearticles values(25.8%); and 1 insert ignore into journalissuearticles values(3.2%);; and of 117 dogs with only tick infestation, 98 insert ignore into journalissuearticles values(83.8%); and 90 insert ignore into journalissuearticles values(76.9%); were positive for E. canis antibodies by IFA test and dot-ELISA, respectively. The agreement between the IFA test and dot-ELISA was 0.73 and was significant insert ignore into journalissuearticles values(P < 0.001);. The specificity and sensitivity of dot-ELISA were calculated to be 96% and 82%, respectively. In conclusion, it was determined that IFA test and dot-ELISA gave concordant results and that both could be used in the serologic diagnosis of ehrlichiosis.
Keywords : Dot ELISA, Ehrlichia canis, IFA, dog