Optimisation of UV Treatment Duration to Induce Haploid Androgenesis in the Nile tilapia (Oreochromis niloticus L.)

Authors : İsmihan KARAYÜCEL, Sedat KARAYÜCEL

Pages : 401-407

View : 19 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The optimum UV duration times of eggs were examined in order to develop a simple and safe method for inducing androgenetic development in the Nile tilapia, Oreochromis niloticus. The yield of androgenetic haploid O. niloticus to the pigmentation stage was 18.53 ± 5.3% insert ignore into journalissuearticles values(relative to controls); with an optimal UV irradiation dose of 540 Jm-2 insert ignore into journalissuearticles values(at 150 µWcm-2 ); for 6 min. Most embryos developing after fertilisation with normal spermatozoa showed abnormal morphology and a haploid number of chromosomes insert ignore into journalissuearticles values(n = 22);. The success of oocyte denucleation was also assessed by using the recessive `blond` skin pigmentation character.
Keywords : Androgenesis, chromosome manipulations, Nile tilapia, Oreochromis niloticus