The Effects of Gonadotrophins on In Vitro Maturation of Cat Oocytes

Authors : Mithat EVECEN, Alper BARAN

Pages : 1315-1320

View : 10 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of this study was to determine the effects of FSH and LH hormones on in vitro development of cat oocytes. The ovaries from spayed cats were brought to the laboratory in +4 °C PBS solution two hours after being collected. The oocytes were recovered by slicing of ovaries and were separated into two groups: Group 1 insert ignore into journalissuearticles values(Control);; TCM 199 + 5% FCS + 2.2 g/l NaHCO3 + 0.23 mM Na Pyruvate; and Group 2 insert ignore into journalissuearticles values(Treatment);; TCM 199 + 5% FCS + 2.2 g/l NaHCO3 + 0.23mM Na Pyruvate + FSH insert ignore into journalissuearticles values(Sigma F-2293); insert ignore into journalissuearticles values(1 µg/ml); + LH insert ignore into journalissuearticles values(Sigma L-5269); insert ignore into journalissuearticles values(1 µg/ml);. Oocytes were matured for 48 hours at 38.5 °C and 5% CO2 in a humidified atmosphere. At the end of this period oocytes were fixed, stained and evaluated for maturation criteria by phase-contrast microscope at x400 magnification. Data were analyzed statistically by Student`s t - test. A total of 428 oocytes insert ignore into journalissuearticles values(Control: 203; Treatment: 225); were used. Eleven of the control group oocytes were at M I insert ignore into journalissuearticles values(5.4%);, 2 insert ignore into journalissuearticles values(0.98%); were at anaphase I and 2 insert ignore into journalissuearticles values(0.98%); were at M II stage. These values for the treatment group were 25 insert ignore into journalissuearticles values(11.11%);, 0 insert ignore into journalissuearticles values(0.00%); and 6 insert ignore into journalissuearticles values(2.66%);, respectively. There was no statistical difference between the control and hormone added groups insert ignore into journalissuearticles values(p>0.05);. For the oocytes which continued to undergo meiosis, the difference between the groups insert ignore into journalissuearticles values(Control: 22.66%; Treatment: 31.55%); was statistically significant insert ignore into journalissuearticles values(p
Keywords : Cat, Oocyte, In Vitro Maturation, Gonadotrophins