Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage

Authors : Aykut ÖZKUL, Kadir YEŞİLBAĞ, İbrahim BURGU

Pages : 1043-1048

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this paper, four diagnostic techniques were compared in terms of their ability to detect BVD virus in buffy coats frozen for more than one year. The samples were selected according to their initial test results. Forty-five BVDV positive and 15 BVDV negative buffy coats were used. The techniques used were the virus isolation-immunoperoxidase test insert ignore into journalissuearticles values(IPX);, immunoperoxidase monolayer assay insert ignore into journalissuearticles values(IPMA);, indirect immunoperoxidase monolayer assay insert ignore into journalissuearticles values(IIPMA); and reverse transcription-polymerase chain reaction insert ignore into journalissuearticles values(RT-PCR);. All BVDV negative samples from previous studies were also negative on the tests used in this study. Out of 45 BVDV-positive samples, 42 insert ignore into journalissuearticles values(93.3%); were found to be positive with RT-PCR, 41 insert ignore into journalissuearticles values(91.1%); with virus isolation-IPX, 40 insert ignore into journalissuearticles values(88.8%); with IIPMA and 37 insert ignore into journalissuearticles values(82.2%); with IPMA. The results obtained indicated that RT-PCR is more suitable for the detection of BVD virus in frozen buffy coats stored for more than one year when compared to other techniques, and IIPMA can be used for the routine diagnosis of BVDV infection as an alternative technique to virus isolation-IPX.
Keywords : BVDV, diagnosis, immunoperoxidase, RT PCR