Characterisation of an Exopolysaccharide Preventing Phage Adsorption in Lactococcus lactis subsp. cremoris MA39

Authors : Mustafa AKÇELİK, Pınar ŞANLIBABA

Pages : 1151-1156

View : 15 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Lactococcus lactis subsp. cremoris strain MA39 produces an extracellular polysaccharide containing rhamnose, glucose and galactose. A 16.5 kb plasmid encoding exopolysaccharide production in MA39 was determined by plasmid curing experiments. Phage adsorption assays showed that four different lactococcal phages were adsorbed to MA39-40; only the 16.5 kb plasmid cured an exopolysaccharide non-producing mutant of strain MA39, with high efficiency insert ignore into journalissuearticles values(93.2-98.5%); while adsorption of these phages was completely inhibited in the wild type strain MA39. These results suggest that exopolysaccharide prevents phage adsorption by masking phage receptor sites.
Keywords : Exopolycaccharide, phage resistance, Lactococcus lactis subsp Cremoris