Detection and RFLP Analysis of Canine Parvovirus (CPV) DNA by Polymerase Chain Reaction (PCR) in a Dog

Authors : Aykut ÖZKUL

Pages : 1201-1203

View : 14 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, the detection of canine parvovirus insert ignore into journalissuearticles values(CPV); in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction insert ignore into journalissuearticles values(PCR); in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm insert ignore into journalissuearticles values(RFLP); technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.
Keywords : Canine parvovirus, diagnosis, PCR, RFLP