Comparison and Evaluation of the Passive Haemagglutination Test and ELISA in Routine Screening of Contagious Equine Metritis (CEM) in Mares with Endometritis or Infertility

Authors : N. Yakut ÖZGÜR

Pages : 989-994

View : 16 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :One hundred and twenty thoroughbred breeder mares with endometritis or infertility were investigated for the detection of antibodies to Taylorella equigenitalis by the passive haemagglutination insert ignore into journalissuearticles values(PHA); test and ELISA between 1997 and 1999 in the Marmara region and the two tests were compared. Fifty-eight insert ignore into journalissuearticles values(48.33 %); of 120 sera were found to be positive by the PHA test and 52 insert ignore into journalissuearticles values(43.33%); by ELISA. 22 of the sera were found to be positive by the PHA test reacted at 1/64 titer, 27 at 1/128, 7 at 1/256 and 2 at 1/512 titer. Sera from 44 mares were positive in both tests. Amongst these, 14 sera were positive in the PHA test and 8 sera were positive in ELISA. The difference between the PHA test and ELISA positivity was 8% and this was not statistically significant. In conclusion, the PHA test was recommended for routine screening of CEM because of its easy handling and rapidness.
Keywords : Taylorella equigenitalis, Contagious equine metritis, passive haemagglutination test, ELISA, infertility, endometritis