Rapid Identification of Avian Salmonella Isolates by Air Thermal Cycler Amplification and Capillary Gel Electrophoresis

Authors : K. Tayfun ÇARLI, Vildan CANER

Pages : 717-720

View : 12 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The possible use of a genus-specific polymerase chain reaction insert ignore into journalissuearticles values(PCR); for the identification of Salmonella colonies was tested on 14 reference strains and 38 clinical Salmonella strains isolated from chickens by the standard method of the National Poultry Improvement Plan and Auxiliary Provisions insert ignore into journalissuearticles values(NPIP);, USDA, U.S.A. All the PCRs using primer pairs InvA1 and InvA2 insert ignore into journalissuearticles values(5); gave 281 bp amplification product specific for the Salmonella genus and produced results identical insert ignore into journalissuearticles values(100%); to those obtained with biochemical and serological methods. Thus, the genus-specific capillary PCR for the identification of a colony of Salmonellae from a selective agar plate was performed within approximately 2 h and the total time required for definitive diagnosis of infection was 2 days using primary enrichment insert ignore into journalissuearticles values(PE); and 7 days using delayed secondary enrichment insert ignore into journalissuearticles values(DSE);.
Keywords : Salmonella, poultry, polymerase chain reaction