Determination of Serum and Urine Proteins by Electrophoresis and Its Importance in Clinical Veterinary Medicine

Authors : Arif ALTINTAŞ

Pages : 93-104

View : 13 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, for the investigation of routine clinical usage of urine protein electrophoresis in the laboratory to compare with the clinical findings in veterinary medicine, qualitative and quantitative changes in protein patterns of serum and urine from dogs with kidney disorders and proteinuria were investigated using electrophoresis. Eighteen dogs insert ignore into journalissuearticles values(patient group);of various ages and breeds with kidney disorders proved in the clinic and the laboratory and seventeen healthy dogs insert ignore into journalissuearticles values(control group);were used in this study. The histories insert ignore into journalissuearticles values(age,sex,breed);of the dogs in the control and patient groups were recorded in the clinic.The blood was collected from the cephalic vein,and the serum was removed using a centrifuge.Urine was obtained by a sterile catheter from the ureter.Biochemical analysis of urine was performed in the clinic.The urines of dogs with proteinuria were centrifuged and ultrafiltrated to concentrate for protein electrophoresis. Serum and urine proteins were separated by 10 %SDS-PAGE. Creatinine in serum and urine was detected using the Jaffe reaction, and urea and total protein in serum by the modified Gentzkow and biuret methods respectively. A/G ratios in serum and protein/creatinine ratios in urine were calculated arithmetically. Levels of serum urea and creatinine were found to be high and statistically significant insert ignore into journalissuearticles values(p
Keywords : Dog, Kidney disorder, Proteinuria, SDS PAGE, Urine, Serum