A Mouse Model for Evaluating the Induction of Liver Glucose-6- Phosphate Dehydrogenase by Halothane

Authors : Ergül Belge KURUTAŞ

Pages : 511-515

View : 8 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The effect of halothane anesthesia in different doses and a period of two weeks on glucose-6-phosphate dehydrogenase insert ignore into journalissuearticles values(G6PDH); activity of mouse liver were investigated. The mean and SD of G6PDH for liver of normal mice were 0.036±0.017 U/mg protein and 1.756±0.814 U/g liver, respectively. Although there was histopathological damage in the liver, liver weights in treated mice increased significantly compared to control animals. Increasing the dosage of halothane inhalation induced liver G6PDH enzyme activity insert ignore into journalissuearticles values(U/g liver); by 84%, 42% and 4%, showing an inverse relation with the dose of halothane insert ignore into journalissuearticles values(0.25, 0.50, 1.00 cc twice daily for two weeks);. Although the Michaelis constant insert ignore into journalissuearticles values(Km); of the partially purified enzyme for glucose-6-phosphate insert ignore into journalissuearticles values(G6P); and nicotinamide adenine dinucleotide phosphate insert ignore into journalissuearticles values(NADP); were similar, the utilization of analogs as 2-deoxyglucose-6-phosphate insert ignore into journalissuearticles values(2d- G6P);, galactose-6-phosphate insert ignore into journalissuearticles values(Gal-6P); and nicotinamide adenine dinucleotide insert ignore into journalissuearticles values(NAD); were different. The induced enzyme was more heat stable than the control.
Keywords : Halothane, G6PDH, mouse liver, induction, kinetic properties