Some Properties of Purified and Non-purified Rumen Tissue Arginase in Cattle

Authors : Mine ERİŞİR, Sema Temizer OZAN

Pages : 597-608

View : 17 | Download : 3

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Some biochemical properties of purified and non-purified rumen tissue arginase were compared. Homogenization, heating, treatment with aceton, precipitation with ammonium sulfate, dialysis, several centrifugations, gel filtration on sephadex G-200 processes were utilized in the purification procedure of the enzyme. It was found that pre-incubation temperature insert ignore into journalissuearticles values(60 °C); of arginase and Km insert ignore into journalissuearticles values(4mM); to its substrate, L-arginine, did not change before and after purification. While pre-incubation period was 10 min and optimal pH 9.7 before purification, pre-incubation period was found to be 5 min and optimal pH 10 after purification. Purified enzyme achieved its highest activity at 2 mM MnCl2 concentration, whereas non-purified enzyme gave the highest activity at 1 mM MnCl2 concentration. Consequently, it was determined that Mn++ cations and preincubation were essential for the activation of the enzyme.
Keywords : Cattle Rumen Tissue, Arginase, Purification, Kinetic properties