Effects of Different Maturation Periods on In Vitro Maturation of Bovine Oocytes

Authors : Sema BİRLER, Serhat PABUÇÇUOĞLU, İ. Kamuran İLERİ, Serhat ALKAN, Mithat EVECEN

Pages : 551-558

View : 12 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Primer oocytes insert ignore into journalissuearticles values(n=157); collected from ovaries of slaughtered cows were matured at 39°C with 95-100% humidity and under a gas mixture of 5% O 2 , 5% CO 2 and 90% N 2 for 22 insert ignore into journalissuearticles values(n=52);, 24 insert ignore into journalissuearticles values(n=52); and 26 insert ignore into journalissuearticles values(n=53); hours. Modified Parker`s Medium insert ignore into journalissuearticles values(MPM); supplemented with FSH and 20% ECS was used as maturation medium. At the end of these periods all oocytes were fixated in ethanol:acetic acid in the ratio of 3:1 for 24 hours and stained by %2 aceto-orcein to evaluate the maturational criteria. At the 22, 24 and 26-hours maturation groups, 23insert ignore into journalissuearticles values(%44.2);, 27 insert ignore into journalissuearticles values(%51.9); and 30 insert ignore into journalissuearticles values(%56.6); oocytes reached the metaphase I and II stages, respectively. The difference among the groups was not important statistically insert ignore into journalissuearticles values(p>0.05);.
Keywords : Bovine, in vitro maturation, maturation time