Melatonin receptor 1A gene RsaI and inhibin alpha subunit gene HaeII polymorphisms in Honamli and Hair goat breeds reared in Western Mediterranean region of Turkey

Authors : Özgecan KORKMAZ AĞAOĞLU, Mustafa SAATCI, Bilal AKYÜZ, Özkan ELMAZ, Mehmet ÇOLAK, Burcu Menekşe BALKAN, Emel ZEYTÜNLÜ

Pages : 23-28

Doi:10.3906/vet-1409-31

View : 14 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The melatonin receptor 1A insert ignore into journalissuearticles values(MTNR1A); and inhibin alpha subunit insert ignore into journalissuearticles values(INHA); genes play a significant role in the reproductive characteristics of animals. Blood samples were collected from 371 goats insert ignore into journalissuearticles values(Honamli and Hair); reared in Antalya and Burdur. The polymerase chain reaction insert ignore into journalissuearticles values(PCR); products were digested by RsaI for the MTNR1A gene and HaeII for the INHA gene. Two alleles insert ignore into journalissuearticles values(A and G); and three genotypes insert ignore into journalissuearticles values(AA, AG, and GG); were observed for the INHA gene, while two alleles insert ignore into journalissuearticles values(R and r); and two genotypes insert ignore into journalissuearticles values(RR and Rr); were observed for the MTNR1A gene. The highest allelic frequency value for G insert ignore into journalissuearticles values(91.8%); was found in Honamli goat breeds for the INHA gene while the highest value for R insert ignore into journalissuearticles values(98.1%); was found in Hair goat breeds for the MTNR1A gene. The GG genotype for the INHA gene and the RR genotype for the MTNR1A gene were identified as the most common genotypes of the Honamli and Hair goat breeds. The rr genotype for the MTNR1A gene could not be determined in the breeds. Both Honamli and Hair goat breeds were in Hardy–Weinberg equilibrium for the genes that were studied. In conclusion, this study confirms the existence of genetic polymorphism in the MTNR1A and INHA genes as detected by PCR-RFLP analysis in Honamli and Hair goat breeds.
Keywords : Goat, Honamli, Hair, INHA, MTNR1A