Effect of freezing rate on goat sperm morphology and DNA integrity

Authors : Burcu ÜSTÜNER, Zekariya NUR, Selim ALÇAY, Mehmet Berk TOKER, Hakan SAĞIRKAYA, Mustafa Kemal SOYLU

Pages : 110-114

Doi:10.3906/vet-1407-70

View : 13 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 °C to -150 °C insert ignore into journalissuearticles values(G10: 10 °C/min, G12: 12 °C/min, G15: 15 °C/min, and G24: 24 °C/min); and stored in liquid nitrogen until use. Semen samples were examined for sperm motility, defective acrosomes insert ignore into journalissuearticles values(FITC-PSA);, and DNA integrity using TUNEL after dilution with extender A at equilibration and postthaw stage. There was no significant difference among the freezing stages in terms of DNA fragmentation insert ignore into journalissuearticles values(P > 0.05);. DNA integrity was partially affected by the freezing rate. The increase of freezing rate from 10 °C/min to 24 °C/min between +5 °C and -150 °C resulted in higher postthaw DNA damage. The study found that the freeze-thawing process is detrimental to postthawed goat semen motility insert ignore into journalissuearticles values(P < 0.05);, acrosome integrity insert ignore into journalissuearticles values(P < 0.05);, and DNA integrity insert ignore into journalissuearticles values(P > 0.05);. Although the freezing rates used in the present study had no effect on postthaw sperm motility and acrosome integrity insert ignore into journalissuearticles values(P > 0.05);, sperm DNA integrity was affected.
Keywords : DNA integrity, freezing rate, goat semen