Detection of sensitive and mutant ruminal bacteria isolates from sheep, cattle, and buffalo using 14 therapeutic antibiotics

Authors : Abdelfattah Zeidan Mohamed SALEM, Roberto Montes de OCA JIMENEZ, Maria Andrea CERRILLO-SOTO

Pages : 514-519

Doi:10.3906/vet-1402-11

View : 9 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In the present study, sensitive and mutant colonies of some ruminal bacterial species isolated from sheep, cattle, and buffalo were detected. We counted and considered \`mutant colonies\` the bacterial colonies grown in the clear inhibition zone in the Kirby-Bauer disk diffusion susceptibility test. Detected mutant colonies were higher in buffalo than in cattle and sheep. Duricef and metronidazole caused no mutations in any species. The others formed mutant colonies, where roxithromycin = polymyxin = chloramphenicol = gentamicin < erythromycin < vancomycin < piperacillin = cefotaxime < streptomycin < cefoperazone < ciprofloxacin < amikacin. Sheep had the highest number of sensitive isolates, and the number of sensitive isolates was dramatically lower in cattle and buffalo. There were no sensitive isolates with the antibiotic metronidazole, and there was a low number of sensitive isolates with duricef. The other antibiotics had more sensitive isolates insert ignore into journalissuearticles values(gentamicin = ciprofloxacin = amikacin > streptomycin = piperacillin > erythromycin > vancomycin = cefoperazone = cefotaxime > roxithromycin > polymyxin > chloramphenicol);. The number of sensitive isolates of the different ruminant species for all the antibiotics was highest in buffalo, followed by cattle and then sheep insert ignore into journalissuearticles values(P < 0.05);. We could conclude that subtherapeutic antibiotic use in ruminant feeding may lead to the formation of antibiotic-resistant mutant colonies, making their subtherapeutic effect nonexistent.
Keywords : Antibiotics, mutant isolates, resistance, ruminal bacteria