Serotyping and antibiotic susceptibility of Listeria monocytogenes isolated from ready-to-eat foods in Samsun, Turkey

Authors : Göknur TERZİ, Ali GÜCÜKOĞLU, Özgür ÇADIRCI, Tolga UYANIK, Mustafa ALİŞARLI

Pages : 211-217

Doi:10.3906/vet-1407-15

View : 11 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The objective of the present study was to assess the presence of Listeria monocytogenes and to investigate its serotyping and antibiotic resistance in ready-to-eat insert ignore into journalissuearticles values(RTE); foods. A total of 100 RTE foods, including 25 stuffed mussels, 25 ezme samples insert ignore into journalissuearticles values(a Turkish-style tomato dip/condiment);, 25 fried spiced livers, and 25 mayonnaise-based salads were obtained from retail shops and supermarkets in Samsun, Turkey. Samples were analyzed using the standard procedure EN ISO 11290-1 and isolates of L. monocytogenes were confirmed for the presence of the hemolysin gene insert ignore into journalissuearticles values(hylA); by polymerase chain reaction. L. monocytogenes was identified in 1 of 25 insert ignore into journalissuearticles values(4%); mayonnaise-based salads as serotype 4b, 2 of 25 insert ignore into journalissuearticles values(8%); ezme samples as serotype 1/2a, and 1 of 25 insert ignore into journalissuearticles values(4%); fried spiced livers as serotype 4b. None of the stuffed mussel samples were found to be contaminated with L. monocytogenes. Antibiotic resistance profiles of L. monocytogenes isolates were detected by the disk diffusion method. Eight antibiotics disks were selected for resistance tests. One of the 4 isolates of L. monocytogenes was resistant to oxytetracycline and 1 isolate was resistant to vancomycin. In conclusion, the presence of L. monocytogenes serotypes 4b and 1/2a in RTE foods is of significant concern for public health as these serotypes are predominant serotypes that can cause listeriosis in humans.
Keywords : Listeria monocytogenes, ready to eat food, serotyping, antibiotic susceptibility, polymerase chain reaction