Effect of Effective Microorganisms on intestinal morphology and morphometry in Japanese quails

Authors : Michał GESEK, Rajmund SOKÓŁ, Barry D. LAMBERT, İwona OTROCKA-DOMAGAŁA

Pages : 285-291

View : 7 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of this study was to determine the effect of supplementation with Effective Microorganisms insert ignore into journalissuearticles values(EM); on the morphology and the morphometry of the intestine, and on proliferation activity of crypt cells in Japanese quails. The EM group insert ignore into journalissuearticles values(16 birds); received EM for 30 days in water insert ignore into journalissuearticles values(3 L/1000 L of water); and in feed insert ignore into journalissuearticles values(5 kg/1000 kg of feed); mixed with standard diet. The second group insert ignore into journalissuearticles values(16 birds); was established as the control group insert ignore into journalissuearticles values(no EM supplementation);. Histopathological analysis revealed infiltration of lymphoid cells mainly in the duodenum and jejunum similarly in the EM and the control groups. The EM group showed irregular villus surfaces when compared to the control group. Morphometric analysis revealed significant differences in the EM group for villus width in the duodenum insert ignore into journalissuearticles values(186.0 μm to 170.7 μm; P ≤ 0.05);, in crypt depth insert ignore into journalissuearticles values(129.2 ?m to 110.7 μm; P ≤ 0.05);, muscular layer thickness in the jejunum insert ignore into journalissuearticles values(70.88 ?m to 57.44 ?m); insert ignore into journalissuearticles values(P ≤ 0.05);, and muscular layer thickness in the ileum insert ignore into journalissuearticles values(76.88 ?m to 64.76 ?m; P ≤ 0.05);. Immunohistochemical analysis of proliferating cell nuclear antigen activity was similar for the EM and the control groups: in the duodenum 90.82% in the control group and 91.89% in the EM group, in the jejunum 93.32% in the control group and 94.53% in the EM group, and in the ileum 92.53% in the control group and 93.92% in the EM group. Supplementation with EM resulted in improved structure and functions of the villi and changed their surface, height, width, and crypt depth.
Keywords : Effective Microorganisms, histopathology, Japanese quail, morphometry