Comparison of IgG and semiquantitative tests for evaluation of passive transfer immunity in calves

Authors : Onur TOPAL, Hasan BATMAZ, Zafer MECİTOĞLU, Ender UZABACI

Pages : 302-309

View : 11 | Download : 3

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Serum immunoglobulin insert ignore into journalissuearticles values(IgG); and semiquantitative tests are used for the evaluation of passive transfer immunity insert ignore into journalissuearticles values(PTI); in calves. We aimed to evaluate PTI in calves by using a Brix refractometer, total protein insert ignore into journalissuearticles values(TP);, gamma-glutamyl transferase insert ignore into journalissuearticles values(GGT);, and glutaraldehyde coagulation test insert ignore into journalissuearticles values(GCT); on days 1, 3, and 7 after birth; to compare the results with serum IgG; and to evaluate which day these tests are given on will give the best results. The blood samples were collected from 60 Holstein dairy calves on day 0, just after birth, and on the 1st, 3rd, and 7th days after birth. The serum IgG concentration was measured by ELISA, the Brix % and TP concentrations with refractometers, and GGT activity using a dry chemistry system. The duration of the GCT was determined in the first 60 min. The IgG, TP concentration, and Brix % all peaked on the 3rd day of the study. GGT showed a significant decline after 24 h. Evaluating the Brix %, IgG, TP, and GCT levels more than 24 h after birth gives better results. However, GGT activity was observed as an early indicator of failure of passive transfer, as the GGT levels were highest 24 h after birth.
Keywords : Calf, immunity, Brix refractometer, total protein, gamma glutamyl transferase, glutaraldehyde coagulation test