Genetic diversity of different avian strains of Pasteurella multocida recovered during four outbreaks in India

Authors : Kumaragurubaran KARTHIK, Vishwanathan DILLIBABU, Ramalingam MAHAPRABHU, Ramasamy BHARATHI, Kaliyaperumal MANIMARAN, Kulasekaran SHOBA

Pages : 310-318

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :  Pasteurella multocida causes fowl cholera in avian species and type A is predominantly reported from outbreaks of fowl cholera. Biochemical, serological, and molecular methods are employed for its diagnosis and typing. Utilizing rapid molecular tools like repetitive extragenic palindromic insert ignore into journalissuearticles values(REP); PCR and enterobacterial repetitive intergenic consensus insert ignore into journalissuearticles values(ERIC); PCR, P. multocida isolates from four outbreaks insert ignore into journalissuearticles values(three from Chennai and one from Ahmadabad, India); were characterized and typed to determine their relationships. A total of 36 isolates were recovered from the outbreaks, including one isolate from a parakeet, which was also subjected to characterization by conventional and molecular analysis. All of the isolates were found to be capsular type A based on PCR assay capsular typing. ERIC- and REP-PCR showed differences in the banding patterns among different outbreak isolates, and also among the geographical regions. Differences were also noticed among different host species, as the banding pattern in the ERIC- and REP-PCR differed; the analysis of results also revealed the same. All of the isolates were found to be sensitive to enrofloxacin and cefotaxime among the antibiotics used in the study. It was found that different strains might have been involved in the different outbreaks reported in the study. The results show that molecular typing methods like ERIC- and REP-PCR are useful epidemiological tools for classifying the strains.
Keywords : Pasteurella multocida, ERIC PCR, REP PCR, fowl cholera, genotyping