Molecular types and antimicrobial resistance profile of Staphylococcus aureus isolated from dairy cows and farm environments

Authors : Dattatray GANDHALE, Rahul KOLHE, Saba NALBAND, Padmakar DESHPANDE, Urmila JAGTAP, Chandrakant DHANDORE, Sujata BHAVE, Sameer JADHAV, Dushyant MUGLIKAR, Smita KOLHE

Pages : 713-724

View : 8 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :  The aim of this study was to assess the prevalence of Staphylococcus aureus in cows with subclinical mastitis and detect enterotoxin genes and patterns of antimicrobial resistance. Out of 180 animals, subclinical mastitis was detected in 78 cows insert ignore into journalissuearticles values(46.99%); and three buffalo insert ignore into journalissuearticles values(21.43%);. The prevalence of clinical mastitis was low insert ignore into journalissuearticles values(3.61%);. S. aureus could be isolated from mastitic milk insert ignore into journalissuearticles values(67/81);, udder swabs insert ignore into journalissuearticles values(38/81);, floor swabs insert ignore into journalissuearticles values(13/27);, milking machine liner insert ignore into journalissuearticles values(5/8);, and healthy animals- milk insert ignore into journalissuearticles values(12/22);. All the strains were confirmed by PCR targeting amplification of the femA gene. Out of 52 PCR-confirmed S. aureus samples, 17 were enterotoxin-producing strains. The pattern of enterotoxin production was revealed as: seb insert ignore into journalissuearticles values(1.92%);, sec insert ignore into journalissuearticles values(21.15%);, sed insert ignore into journalissuearticles values(7.69%);, and see insert ignore into journalissuearticles values(1.92%);. The pattern of superantigens and methicillin resistance was revealed as: etb insert ignore into journalissuearticles values(1.92%);, tst1 insert ignore into journalissuearticles values(25%);, and mecA insert ignore into journalissuearticles values(3.85%);. Combination of sec and tst1 and the rare distribution of sea , seb , and sed was observed. S. aureus isolates were sensitive to netilmicin insert ignore into journalissuearticles values(85.88%);, amikacin insert ignore into journalissuearticles values(80%);, tobramycin insert ignore into journalissuearticles values(76.47%);, and gentamicin insert ignore into journalissuearticles values(68.24%); and resistant to penicillin insert ignore into journalissuearticles values(91.76%);, oxacillin insert ignore into journalissuearticles values(71.76%);, and kanamycin insert ignore into journalissuearticles values(63.53%);. Antimicrobial resistant genes blaZ , ermB , and tetK were also detected in the isolates. This study provides a baseline for monitoring antimicrobial resistance in S. aureus strains associated with bovine mastitis in India.
Keywords : Bovine mastitis, Staphylococcus aureus enterotoxins, antimicrobial resistance