Identification of Candida strains with nested PCR in bovine mastitis and determination of antifungal susceptibilities

Authors : Göksel ERBAŞ, Uğur PARIN, Şükrü KIRKAN, Serap SAVAŞAN, Mehmet Volkan ÖZAVCI, Hafize Tuğba YÜKSEL

Pages : 757-763

View : 10 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Mastitis is one of the biggest problems of dairy cattle industries. In recent years, fungal agents have been frequently reported among the causative agents for mastitis. Among the fungal agents, Candida species are the most common. This study`s aims were the isolation, identification, and determination of antifungal susceptibility of Candida species causing mycotic mastitis in cattle. A total of 260 mastitic milk samples were collected from different farms. Identification was conducted by rapid diagnostic tests and nested PCR method; 6 different antifungal agents were examined. Candida sp. was detected in 46 insert ignore into journalissuearticles values(17.7%); of the 260 mastitic milk samples. Based on API 20 C AUX and nested PCR test results, 6 different species of Candida were identified. C. tropicalis was the predominant one insert ignore into journalissuearticles values(26.1%);, followed by C. parasilosis insert ignore into journalissuearticles values(21.7%);, C. kefyr and C. krusei insert ignore into journalissuearticles values(17.4% each);, C. rugosa insert ignore into journalissuearticles values(13%);, and C. glabrata insert ignore into journalissuearticles values(4.4%);. A total of 46 strains were confirmed by PCR. According to antifungal test results, the isolates were found to be susceptible to ketoconazole insert ignore into journalissuearticles values(78.3%); and resistant to flucytosine insert ignore into journalissuearticles values(91.3%);, amphotericin insert ignore into journalissuearticles values(82.6%);, miconazole and nystatin insert ignore into journalissuearticles values(73.9%);, and fluconazole insert ignore into journalissuearticles values(69.5%);.
Keywords : Mastitis, nested PCR, antifungal, Candida sp