Estradiol 17 beta-dehydrogenase 5 is involved in aflatoxin B1 transportation

Authors : Yanling YANG, Yaling HUANG, Linmao ZENG, Yuanyuan GAO, Yu LI, Yanguang JI, Shihua WANG, Kunzhi JIA, Zhenhong ZHUANG

Pages : 482-489

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Numerous studies have focused on the mechanism of aflatoxin B1 insert ignore into journalissuearticles values(AFB1); metabolism and its carcinogenic mechanism, but how AFB1 is transported into hepatocytes and how it is transferred inside hepatocytes remains unknown. In this study, the AFB1-interacting protein, estradiol 17 beta-dehydrogenase 5 insert ignore into journalissuearticles values(Akr1c6);, was identified with an immobilized affinity chromatography technique and LC-MS/MS. The interaction between Akr1c6 and AFB1 was confirmed with ELISA, and the results showed that Akr1c6 could efficiently bind AFB1. Anti-Akr1c6 polyclonal antibody from rabbit was prepared, and the IC50 values of AFB1 to BRL insert ignore into journalissuearticles values(normal big rat liver cells); and NRK insert ignore into journalissuearticles values(normal rat kidney cells); were detected using the MTT assay. It was found that the Akr1c6 expression level in BRL was significantly affected under the IC50 value of AFB1 insert ignore into journalissuearticles values(P < 0.05);, but no obvious expression difference of Akr1c6 was observed in NRK. This suggested that Akr1c6 in liver cells participates in the transportation and/or metabolism of AFB1, and though Akr1c6 was expressed in the kidneys, it did not play a role in AFB1 transportation or metabolism. The conclusions of this study lay a foundation for further exploring the role of AFB1 binding proteins in the toxicology of AFB1 to hepatocytes and the pathway through which AFB1 enters hepatocytes and their nuclei.
Keywords : Aflatoxin B1 binding protein, estradiol 17 beta dehydrogenase 5, toxicology of AFB1, hepatocytes