Field investigation of Trypanosoma evansi and comparative analysis of diagnostic tests in horses from Bahawalpur, Pakistan

Authors : Sonia TEHSEEN, Nusrat JAHAN, Marc DESQUESNES, Mirza İmran SHAHZAD, Muhammad Fiaz QAMAR

Pages : 288-293

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :  In order to assess the prevalence of Trypanosoma evansi , a parasitological, molecular, and serological-based investigation was carried out in randomly sampled horses insert ignore into journalissuearticles values(n = 375); belonging to different age groups, sexes, and localities from the district of Bahawalpur, Pakistan. The diagnostic performance of applied tests was also compared. The prevalence was recorded as 0.5% with Woo`s test, 1.3% with both ITS CF/BR PCR and RoTat 1.2 PCR, and 14.4% with CATT/ T.evansi . Based on CATT/ T.evansi , significant differences insert ignore into journalissuearticles values(P ≤ 0.05); were observed for prevalence estimates according to different localities, sexes, body condition scores, and origins. A nonsignificant difference insert ignore into journalissuearticles values(P ≥ 0.05); was observed among different age groups and variable parity numbers. Our study declares district Bahawalpur to be a high risk area for surra and proposes the potential use of CATT/ T.evansi as a field test of choice for surveys in horses. However, the status of seropositive animals should be confirmed using a more sensitive molecular tool such as the satellite DNA target. A widespread status of anti- Trypanosoma antibodies calls for control measures and further investigation of various species insert ignore into journalissuearticles values(camels, cattle, sheep, goats, buffaloes); inhabiting the same area to identify reservoir status.
Keywords : Horses, Trypanosoma evansi, Bahawalpur