Antimicrobial resistance profiles and virulence factors of Escherichia coli O157 collected from a poultry processing plant

Authors : Harun HIZLISOY, Serhat AL, Nurhan ERTAŞ ONMAZ, Yeliz YILDIRIM, Zafer GÖNÜLALAN, Kadir Semih GÜMÜŞSOY

Pages : 65-71

View : 29 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aims of this study were to investigate the presence of  Escherichia coli  O157 and to determine the antibiotic susceptibility of isolates in different poultry-originated materials. In total 300 samples of chicken carcass swabs insert ignore into journalissuearticles values(n = 50);, chicken feces insert ignore into journalissuearticles values(n = 50);, gizzard insert ignore into journalissuearticles values(n = 50);, liver insert ignore into journalissuearticles values(n = 50);, water in the final scald tank insert ignore into journalissuearticles values(n = 50);, and equipment such as tables, conveyers, and knives insert ignore into journalissuearticles values(n = 50); were used as materials. Overall, 45 E. coli isolates were obtained from the 300 poultry samples. However, 13 insert ignore into journalissuearticles values(4.3%); of the samples were identified as E. coli O157 including 3 liver, 3 carcass, and 7 fecal samples. All 13 isolates were positive for O157 but negative for H7 using PCR. Only intimin insert ignore into journalissuearticles values(encoded by eaeA gene); was detected in 10 E. coli O157 isolates while no other virulence factors insert ignore into journalissuearticles values(enterohemolysin A, shiga toxin 1 and 2); were detected. All E. coli O157 isolates were susceptible to gentamicin insert ignore into journalissuearticles values(100%);. In conclusion, the presence of E. coli O157 in poultry is of utmost significance insert ignore into journalissuearticles values(4.3%); and our results demonstrated that poultry carcasses and giblets may be contaminated by E. coli O157 with feces during evisceration. To prevent E. coli O157 infections, hygiene rules must be tightly observed. In addition, antibiotic use must be controlled for avoiding increased antibiotic resistance.
Keywords : Escherichia coli O157, poultry, virulence, antibiotic resistance