Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India

Authors : Fateh SINGH, Ganesh Gangaram SONAWANE, Jyoti KUMAR, Shivendra Kumar DIXIT, Rajendra Kumar MEENA, Bhupendra Nath TRIPATHI

Pages : 754-760

View : 13 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The present study was planned to isolate Escherichia coli from pneumonic and septicemic sheep and goats and to determine antimicrobial resistance insert ignore into journalissuearticles values(AMR); patterns of the isolates and evaluate them for the presence of extended-spectrum ß-lactamase insert ignore into journalissuearticles values(ESBL); using the phenotypic and molecular methods. Pneumonic lung tissues and heart blood samples were collected by performing necropsy on sheep insert ignore into journalissuearticles values(n = 96); and goats insert ignore into journalissuearticles values(n = 08);. The samples were processed and used for bacterial isolation. E. coli isolates insert ignore into journalissuearticles values(n = 58); including 53 from 34 sheep and 5 from 3 goats were recovered and identified by the cultural and biochemical characteristics and 16S rRNA sequencing. The isolates were tested to ascertain AMR pattern, plasmid features, and ESBL production. The highest rate of resistance insert ignore into journalissuearticles values(65.5%); was recorded against amoxicillin, enrofloxacin, norfloxacin, and ofloxacin. Eighteen insert ignore into journalissuearticles values(31%); out of 58 isolates showed the ESBL phenotype by combination disk assay. There was a variability in the number insert ignore into journalissuearticles values(1-5); and size insert ignore into journalissuearticles values(2 to >20 kb); of plasmids among isolates. Polymerase chain reaction insert ignore into journalissuearticles values(PCR); amplification revealed the presence of blaH$_{TEM}$ insert ignore into journalissuearticles values(70.6%); and blaH$_{SHM}$insert ignore into journalissuearticles values(1.7%); genes. PCR specificity was confirmed by nucleotide sequencing. The present study indicates that the extraintestinal and multidrug-resistant insert ignore into journalissuearticles values(MDR); E. coli strains were associated with pneumonia and/or septicemia in sheep and goats. Therefore, thorough surveillance and monitoring of MDR bacteria are urgently needed to implement the infection control strategies and to restore the efficacy of available antibiotics.
Keywords : Escherichia coli, extended spectrum ß lactamase, multidrug resistance, pneumonia, sheep, septicemia