Development of spermatogonial stem cell niche and immunoexpression of vimentin filaments in the testes of prenatal and postnatal Indian buffalo (Bubalus bubalis)

Authors : Ashritha Quanisha CARLO, Devendra PATHAK, Opinder SINGH, Neelam BANSAL

Pages : 817-824

View : 19 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, we established the components of the buffalo spermatogonial stem cell insert ignore into journalissuearticles values(SSC); niche in prenatal insert ignore into journalissuearticles values(38.5-280 days of gestation);, neonatal, prepubertal, and adult testes histochemically and immunohistochemically. Immunohistochemically, the changes in vimentin expression were evaluated. During prenatal life, the niche of primordial germ cells was formed by the surrounding mesenchymal cells and fibroblasts. The basement membrane insert ignore into journalissuearticles values(BM); was not organized at this stage. At 7.6 cm crown-rump length, testicular cords with a thin BM were seen. At the late gestational period, testicular cords with well-organized BM were seen. At the neonatal stage, the testicular cords were lined with distinct BM, while during the prepubertal period, distinct BM with peritubular myoid cells insert ignore into journalissuearticles values(PMCs); were seen. In adult testes, the seminiferous tubules were surrounded by a thick BM surrounded by PMCs. PM cells were only single-layered as seen in H&E-stained paraffin sections, picrosirius red-stained, and Masson?s trichrome-stained paraffin sections. Collagen fibers were seen to increase quantitatively to provide strength and cushioning to the developing niche of spermatogonial stem cells in the testes. The expression of vimentin increased to a certain extent until adult life, indicating its significant role as an intermediate filament during development.
Keywords : Buffalo, spermatogonial stem cell niche, prenatal, postnatal, testis, vimentin