Pistacia atlantica gum aqueous extract modulates humoral and cellular immune responses in BALB/c mice

Authors : Hadi ESMAEILI GOUVARCHIN GHALEH, Zaynab SHAKARAMI GANDABEH, Bahman MANSOURI MOTLAGH, Ali SHEIKHIAN

Pages : 89-95

View : 10 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Natural herbal products contain different compounds that can modulate immune and inflammatory diseases. This investigation aimed to examine the influence of Pistacia atlantica aqueous extract on cellular and humoral immunity in mice following the exposure to sheep red blood cells insert ignore into journalissuearticles values(SRBCs);. For this purpose, male BALB/c mice insert ignore into journalissuearticles values(n = 40); were randomly assigned into 4 equivalent groups. The animals received 1 × 109 SRBCs in complete Freund?s adjuvant insert ignore into journalissuearticles values(CFA); intraperitoneally 3 times with two-week intervals. Five days after the final injection, blood samples were taken from the animals. Additionally, 1 × 109 SRBCs were also injected into the mice?s left hind footpad 48 h before the blood collection. Three groups of mice received various oral doses of the extract insert ignore into journalissuearticles values(100, 200, and 400 mg/kg); for 52 consecutive days after the study onset. Measurements were performed on the levels of humoral and cellular immune responses, splenocytes? ability to uptake neutral red, respiratory burst, and cell viability. The mice receiving Pistacia atlantica in all concentrations displayed significantly low delayed type hypersensitivity insert ignore into journalissuearticles values(DTH); reactions in a dose-dependent manner compared to the control animals. The treated mice also presented a dose-dependent, significantly lower average anti-SRBC titer than that in the control animals. IL-17 and IL-10 cytokine levels respectively decreased and increased significantly in the mice that received Pistacia atlantica compared to the control group. Splenocyte proliferation and respiratory burst declined significantly in mice treated with Pistacia atlantica as opposed to the normal control group but phagocytosis ability was significantly decreased. The immunomodulatory impacts of Pistacia atlantica might be partially caused by immunity diversion from proinflammatory cytokine IL-17 into antiinflammatory cytokine IL-10.
Keywords : Pistacia atlantica, humoral and cellular immunity, immunomodulation, BALB c mice