Histopathological evaluation of uterine structure and immunohistochemical examination of MMP-1 and TIMP-1 in cows recovered from metritis

Authors : Serdal KURT, Thomas WITTEK, Funda EŞKİ

Pages : 19-25

Doi:10.47748/tjvr.1162968

View : 9 | Download : 4

Publication Date : 2023-03-15

Article Type : Research Paper

Abstract :Objective: The aim of this study is to compare the results of histopathological examination of the uterus of healthy cows and the uterus of cows that have recovered from metritis. Materials and Methods: The study materials obtained from the slaughterhouse were divided into two groups: the uterus of healthy cows insert ignore into journalissuearticles values(no metritis; Group N; n=10); and uterus of cows that had recovered from metritis about 45 days ago insert ignore into journalissuearticles values(metritis; Group M; n=10);. Sections were taken from the uterus of both groups and were stained with hematoxylin and eosin insert ignore into journalissuearticles values(H&E); for pathological comparison. In addition, the sections were stained immunohistochemically for the examination of Matrix metalloproteinases 1 insert ignore into journalissuearticles values(MMP-1); and the tissue inhibitor of matrix metalloproteinases insert ignore into journalissuearticles values(TIMP-1); levels. Results: The uterus showed pathological condition in Group M than in Group N. MMP1 immunopositivity was higher in the luminal epithelium insert ignore into journalissuearticles values(p<0.01);, endometrial stroma, uterine gland insert ignore into journalissuearticles values(p<0.05); and myometrium sections insert ignore into journalissuearticles values(p<0.01); of the uterus in Group M compared to Group N. TIMP-1 immunopositivity in endometrial stroma, uterine gland and myometrium sections increased in Group M compared to Group N insert ignore into journalissuearticles values(p<0.01);. Conclusion: In conclusion, it was observed that the negative effects of metritis on the uterus persisted histologically even approximately 45 days after clinical recovery. However, this needs to be investigated extensively because time elapsed between the clinical recovery of the cows and the study may have been insufficient for the uterus to complete histological healing.
Keywords : Cow, metritis, MMP 1, TIMP 1, uterus