Chemometric Approach Based on Factorial and Box-Behnken Designs for Determination of Anti Coronavirus Drug; Favipiravir in Bulk and Spiked Human Plasma by Green HPLC Method

Authors : Safa MEGAHED, Ahmed HABİB, Sherin HAMMAD, Amira KAMAL

Pages : 70-78

Doi:10.51435/turkjac.963652

View : 17 | Download : 7

Publication Date : 2021-12-30

Article Type : Research Paper

Abstract :The present work describes development of robust, sensitive and green HPLC method with fluorescence detection for determination of favipiravir insert ignore into journalissuearticles values(FAV);. Fractional factorial design was implemented for screening of different factors affecting chromatographic responses. Box-Behnken design was applied to study and optimize the most critical method parameters. The optimum chromatographic conditions obtained involved the use of 0.1% phosphoric acid solution and isopropanol in the ratio 98:2 % v/v as mobile phase at a flow rate of 0.8 mL/min and column oven temperature of 35ºC. Chromatographic analysis was performed on Eclipse plus® C18 insert ignore into journalissuearticles values(100 mm×4.6mm ×3.5 μm); column with fluorescence detector set at 361 nm and 432 nm for excitation and emission, respectively. A linear response was obtained over the range of 20 - 240 ng/mL with limit of detection of 2.01 ng/mL and quantitation limit of 6.11 ng/mL. The method was successfully implemented for determination of FAV in its pharmaceutical formulation with mean % recovery ± SD of 99.42 ± 0.59. Moreover, the sensitivity of the method allowed determination of FAV in spiked human plasma over a range of 40 – 240 ng/mL. The combined application of green chemistry and quality by design leads to development of robust green method.
Keywords : Favipiravir, COVID 19 virus, Box Behnken design, Green chemistry, Human plasma