The Relationship Between CACNA2D1 Gene and Subclinic Mastitis in Holstein Breed Cattle

Authors : Fadime DALDABAN, Korhan ARSLAN, Bilal AKYÜZ

Pages : 93-97

Doi:10.36483/vanvetj.1242270

View : 72 | Download : 98

Publication Date : 2023-07-29

Article Type : Research Paper

Abstract :The CACNA2D1 gene encodes the CACNA2D1 protein and, this protein is involved in the excitation-contraction mechanism of the muscle cells during milk withdrawal, helps the nipples to open and close. Because of this role in physiological mechanism and its relationship with quantitative trait locus insert ignore into journalissuearticles values(QTL); regions, CACNA2D1 gene is known to be associated with mastitis resistance. In this study, it was aimed to investigate the relationship between different three SNP insert ignore into journalissuearticles values(C367400T, A496561G and G519663A); on the CACNA2D1 gene, and subclinical mastitis in Holstein breed cattle reared in Develi district of Kayseri province. SNPs were genotyped from DNA samples by PCR-RFLP method. In the study, California mastitis test insert ignore into journalissuearticles values(CMT); data, and distributions of genotypes of the three SNPs on the CACNA2D1 gene were calculated. In the study, genotype distributions were determined in terms of C367400T, A496561G and G519663A SNPs found on the CACNA2D1 gene according to CMT status. The difference between the C367400T, A496561G and G519663A SNPs was not significant insert ignore into journalissuearticles values(p>0.05);. In the study group examined the Chi-square insert ignore into journalissuearticles values(χ2); analysis conducted, it was observed that the Holstein cattle were in the Hardy-Weinberg equilibrium insert ignore into journalissuearticles values(HWE); in terms of C367400T and A496561G SNPs, deviation from HWE for the G519663A SNP insert ignore into journalissuearticles values(p<0.05);. As a result, it was thought that the CACNA2D1 gene and these SNPs should be evaluated with more samples and different mastitis indicator data in studies on mastitis resistance.
Keywords : CACNA2D1, Genetik belirteç, Sığır, Sığır mastit