First Report of Candida sp. from Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fry in Turkey

Authors : Seçil METİN, Behire İşıl DİDİNEN, Ayşegül KUBİLAY, Bülent BAŞ, Pınar YILDIRIM, Tom WIKLUND

Pages : 55-59

Doi:10.22392/egirdir.437661

View : 22 | Download : 6

Publication Date : 2019-03-01

Article Type : Research Paper

Abstract :In this study, we isolated Candida sp. for the first time from fry of rainbow trout insert ignore into journalissuearticles values( Oncorhynchus mykiss ); in Turkey. Candida species caused disease in fry insert ignore into journalissuearticles values(average 0.2 g); which was observed at 10°C water temperature in February 2016. Due to yeast accumulation on gills, whitish areas was seen and fusion of secondary lamella in gills were observed under microscope. The microbiological samples of kidney, spleen and gills were streaked onto trypticase soy agar insert ignore into journalissuearticles values(TSA); and tryptone yeast extract salts insert ignore into journalissuearticles values( TYES ); agar plates and incubated at 22°C in TSA and 18°C in TYES agar for 48 h. Thirteen isolates were obtained and Gram staining was performed. The all of the isolates were determined as yeast cell based on cellular morphology by using light microscopy . The identification was performed by ITS insert ignore into journalissuearticles values(Internal transcribed spacer); region sequence analysis and sequence data demonstrated that representative isolate D11 belonged to genus Candida . The yeast infection might have seen in early development stages insert ignore into journalissuearticles values(larvae and fry); due to the intensive antibiotic use against rainbow trout fry syndrome insert ignore into journalissuearticles values(RTFS); disease.
Keywords : Candida sp, rainbow trout, Oncorhynchus mykiss, identification, sequence analysis