Phenotypic and iPBS-retrotransposon Marker Diversity in Okra (Abelmoschus esculentus (L.) Moench) Germplasm

Authors : Faik KANTAR, Sevde Nur YEMŞEN, Cansu BÜLBÜL, Neslihan YILMAZ, Nedim MUTLU

Pages : 7-15

Doi:10.38042/biost.2021.30.01.02

View : 17 | Download : 7

Publication Date : 2021-06-15

Article Type : Research Paper

Abstract :This study was undertaken to assess genetic and phenotypic diversity of Turkish okra insert ignore into journalissuearticles values(Abelmoschus esculentus insert ignore into journalissuearticles values(L.); Moench); germplasm of 26 landraces including three cultivars insert ignore into journalissuearticles values(Akköy-41, Kabaklı-11, and Marmara-1); with 34 phenotypic traits and 74 iPBS- retrotransposon primers. Leaf-blade size, fruit length, fruit diameter, fruit number per plant, petiole length, plant height, stem diameter, number of stem nodes, and plant growth type insert ignore into journalissuearticles values(degree of branching); were the most important morphological traits contributing to the variation. Comparison of genotypes with 14 iPBS-retrotransposon primers yielded 141 bands, 34 of which insert ignore into journalissuearticles values(24.1%); were polymorphic, with the primer 2271 producing the highest insert ignore into journalissuearticles values(6); bands per primer. Cluster analysis based on phenotypic and molecular markers produced two major groups. Phenotypic based unweighted pair group method with arithmetic mean insert ignore into journalissuearticles values(UPGMA); dendrogram had 12 sub-groups with the highest similarity insert ignore into journalissuearticles values(0.63); between GAN-19/GAN-21 and MGL-6/Akköy-41 genotypes. The markers, however, produced a dendrogram with eight subgroups, pairwise genetic similarities ranging from 0.43 to 1.00, where MGL-6 singled out with a similarity value of 0.57. Howbeit, the Mantel test between both dendrograms based on the similarity matrix was insignificant.
Keywords : Okra, Landraces, Agro Morphologic, Molecular, Selection