Bazı bitki ekstraktları ve kimyasal maddelerin tütün mozaik virüsü enfeksiyonuna etkilerinin araştırılması

Authors : Neziha ARSLAN, Semih ERKAN

Pages : 189-205

View : 9 | Download : 4

Publication Date : 2012-06-23

Article Type : Research Paper

Abstract :In the present study, the inhibitory effects of 14 plant extracts and 15 chemical substances were examined on the infection of Tobacco mosaic virus insert ignore into journalissuearticles values(TMV);. According to the results of experiments on test plants, plant extracts inhibited virus infection at the ratios of 25.92 % to 97.19 % while the inhibition levels of chemical substances on virus infection ranged between 10.24 % and 98.30 %. The findings showed that the highest inhibition levels were obtained with three plant extracts insert ignore into journalissuearticles values( Dianthus caryophyllus , Capsicum annuum and Yucca elephantipes ); and three chemicals insert ignore into journalissuearticles values(Sodium dodecylsulfate, Triton X-100 and HuwaSan);. Therefore, the mentioned extracts and chemicals were selected for further steps of the study at which the effects of some factors as dilution, temperature and application times on the inhibition of virus infection were examined. Considering the data from assays on test plants, it was observed that inhibitory activity of D. caryophyllus and sodium dodecylsulfate was not affected much more with dilution and temperature fluctuations. Moreover, the results showed that extract from D. caryophyllus had more inhibitory effect when it was applied in 2 and 4 hours before virus inoculation and on the contrary Sodium dodecylsulfate inhibited virus infection at higher level when used in 2 and 4 hours after virus application. Later, in pot experiments performed on tobacco insert ignore into journalissuearticles values( Nicotiana tabacum L. cv. Akhisar 97); plants, it was observed that the application of D. caryophyllus extract and reduction of virus concentration in the tobacco plants by spreying sodium dodecylsulfate before or after virus inoculation was determined by results of DAS-ELISA test.
Keywords : Tütün, tütün mozaik virüsü, engelleme, bitki ekstraktı, kimyasal madde