Investigation of DNA Damage and DNA Repair Capacity in Patients with Colorectal Cancer and Their First Degree Relatives

Authors : Ayfer BECEREN, Gülden Zehra OMURTAG

Pages : 0-0

View : 10 | Download : 6

Publication Date : 2014-01-30

Article Type : Research Paper

Abstract :Objective:  Studies have reported that genetic susceptibility and environmental factors play an important role in the formation of colorectal cancer insert ignore into journalissuearticles values(CRC);. Individuals with incidence of colorectal cancer in the first-degree relatives are about twice as likely to develop CRC as those without any family history. Biomarkers are often utilized for the determination of increased risk of cancer. The aim of our study was to investigate the potential DNA damage using by comet assay and chromosomal aberrations insert ignore into journalissuearticles values(CAs);, measurement of DNA repair capacity by using challenge assay as biomarkers of susceptibility in peripheral lymphocytes of CRC patients and their first degree relatives.  Methods:  Peripheral blood samples were taken from untreated patients diagnosed with CRC insert ignore into journalissuearticles values(n=56);, their first degree relatives insert ignore into journalissuearticles values(n=50); and healthy controls insert ignore into journalissuearticles values(n=25); were analyzed by comet assay and challenge assay and CAs.  Results:  Chromosomal aberration frequency by chromosomal aberration technique in peripheral blood lymphocytes in CRC patients and their first degree relatives were not statistically different as compared with the controls insert ignore into journalissuearticles values(p>0.05);. However, a statistically significant increase in DNA damage was observed by comet assay insert ignore into journalissuearticles values(p<0.001);. Challenge assay demonstrated statistically significant reduction in DNA repair capacity in CRC patients insert ignore into journalissuearticles values(p<0.001); and as in their first degree relatives insert ignore into journalissuearticles values(p=0.001); as compared with the controls.  Conclusion:  These results demonstrate that comet assay can be used as a biomarker for detecting DNA damage in CRC and challenge assay can be utilized clinically for identification of CRC susceptibility in individuals who has relatives with CRC. Anahtar Kelimeler :  DNA damage, colorectal cancer, comet assay, chromosomal aberration, DNA repair capacity
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