Effects of Water Samples in Polyethylene Terephthalate Bottles Stored at Different Conditions on Zebrafish Embryos with Relevance to Endocrine Disrupting Chemical Migration and Adenomatous Polyposis Coli Tumor Suppressor Gene

Authors : Unsal Veli USTUNDAG, İsmail UNAL, Perihan Seda ATES, Aybuke TİRPANCİ, Gizem EGİLMEZER, Ahmet Ata ALTURFAN, Turkan YİGİTBAS, Ebru EMEKLİ ALTURFAN

Pages : 171-177

Doi:10.33808/clinexphealthsci.564016

View : 17 | Download : 10

Publication Date : 2019-06-30

Article Type : Research Paper

Abstract :Objective: Polyethylene terephthalate insert ignore into journalissuearticles values(PET); is a material that is most commonly used for production of clear plastic bottles. Adenomatous polyposis coli insert ignore into journalissuearticles values(APC); and β-catenin have been related with cancer. Aim was to investigate the effects of PET bottled water samples that were exposed to sunlight and hot water on zebrafish embryos. Moreover the effects of these water samples on APC knockdown zebrafish embryos were also evaluated. Methods: Phthalate concentrations in water samples were determined using ELISA. Immunohistochemical method and RT-PCR were used to analyse the expressions of proliferative cell nuclear antigen insert ignore into journalissuearticles values(PCNA);, β catenin, Wnt 3a and Gsk3β. Biochemical parameters were measured using spectrophotometric methods. Vitellogenin concentrations were measured using ELISA and apoptotic cells were evaluated by Acridine Orange staining. Results: Increased PCNA, β-catenin, Wnt 3a, Gsk3β expressions, vitellogenin, nitric oxide, apoptosis and impaired oxidant-antioxidant balance were observed in the exposure groups with these increases being more profound in APC knockdown groups. Conclusion: APC knockdown embryos were more prone to the deleterious effects of water samples used in this study.
Keywords : Polyethylene terephthalate, phthalates, Wnt β catenin signaling, adenomatous polyposis coli