Cytotoxicity and Collagen Expression Effects of Tideglusib Administration on Human Periodontal Cells: An In-Vitro Study

Authors : Buse ONCU, Ayse MINE YILMAZ, Betül KARADEMİR YILMAZ, Elif Çiğdem ALTUNOK, Leyla KURU, Ömer Birkan AĞRALI

Pages : 473-473

View : 20 | Download : 13

Publication Date : 2020-12-30

Article Type : Other Papers

Abstract :Objective: Tideglusib is a GSK-3 inhibitor activating Wnt/β-catenin signaling pathway which has significant importance in regenerative response. The aim of this study was to evaluate the cytotoxicity and protein expression impacts of Tideglusib on human periodontal cell lines. Methods: Cytotoxicity effect of different concentrations insert ignore into journalissuearticles values(50nM, 100nM, 200nM); of Tideglusib application on human gingival fibroblast insert ignore into journalissuearticles values(hGF);, periodontal ligament fibroblast insert ignore into journalissuearticles values(hPDLF);, and osteoblast insert ignore into journalissuearticles values(hOB); cell lines was determined. Type-I and III collagen expressions were evaluated after 24-hour application of 50nM Tideglusib. Results: The cytotoxicity of 200nM Tideglusib was higher in hGF and hOB insert ignore into journalissuearticles values(p<0.05);, but no difference was found in hPDLF compared to the respective control group insert ignore into journalissuearticles values(p>0.05);. The hGF and hOB treated with 50nM Tideglusib expressed an increased level of Type-I collagen insert ignore into journalissuearticles values(p<0.05);, but no difference was detected in the hPDLF compared to the respective control insert ignore into journalissuearticles values(p>0.05);. Type-III collagen expressions were similar between the test and control groups for each cell line insert ignore into journalissuearticles values(p>0.05);. Conclusion: Tideglusib is not cytotoxic at 50nM and 100nM concentrations and may have positive effect on bone regeneration rather than periodontal regeneration since it stimulated Type-I collagen production in hGF and hOB cells, but not in hPDLF.
Keywords : Cell biology, , cell signaling biomolecules, osteoblast s, , tideglusib, Wnt β catenin signaling pathway