POTENTIAL BIOMARKERS FOR EARLY DETECTION OF ORAL CANCER: LEPTIN AND ADIPONECTIN

Authors : Nezaket Ezgi ÖZER, Aslı TETİK, Betül KARACA, Ali VERAL, Pelin GÜNERİ

Pages : 21-24

Doi:10.52037/eads.2022.0016

View : 14 | Download : 6

Publication Date : 2022-08-31

Article Type : Other Papers

Abstract :Aim: To determine adiponectin and leptin expression levels in samples from healthy, dysplastic and cancerous oral lesions and to investigate the potential role of these biomarkers in oral cancer development. Methods: Tissue samples from the archives which were histologically diagnosed as oral squamous cell carcinoma insert ignore into journalissuearticles values(OSCC); insert ignore into journalissuearticles values(n=24);, oral dysplasia insert ignore into journalissuearticles values(n=12); and healthy tissue insert ignore into journalissuearticles values(n=16); were included. The concentration and purity of samples were determined after total RNA extraction. Thereafter, cDNA synthesis was performed. The differences between expression levels were evaluated with qPCR. Expression levels related to OSCC were performed according to normalizing gene ACTB. 2-ΔΔCT method was used for comparison of expression levels. Student t-test was used to compare the differences in gene expression levels between groups, using 2-ΔΔCT value for each gene. Results: Adiponectin and leptin expression levels were decreased by 1.09 insert ignore into journalissuearticles values(p = 0.49); and 10.3 insert ignore into journalissuearticles values(p = 0.22);, respectively, in OSCC group, however differences were not statistically significant. The level of adiponectin expression decreased by 1.65 times insert ignore into journalissuearticles values(p = 0.62); and leptin expression increased by 3 times insert ignore into journalissuearticles values(p = 0.30); in the dysplasia group. When OSCC and dysplasia groups were compared, it was found that the level of adiponectin expression increased by 1.52 times insert ignore into journalissuearticles values(p = 0.99); and the level of leptin expression decreased by 30.94 times insert ignore into journalissuearticles values(p = 0.16); in the OSCC group. The samples were re-grouped as increased risk insert ignore into journalissuearticles values(OSCC+dysplasia); insert ignore into journalissuearticles values(n=36); and healthy insert ignore into journalissuearticles values(n=16); and a decrease by 16.5 insert ignore into journalissuearticles values(p= 0.3); in leptin expression and an increase by 1.14 insert ignore into journalissuearticles values(p= 0.6); in adiponectin expression was observed in the increased risk group compared to the healthy group. Conclusion: In tissue samples diagnosed as healthy, dysplastic and OSCC, expression levels for leptin and adiponectin have not been compared before. The results revealed a significant decrease in leptin expression and an increase in adiponectin expression in increased risk group.
Keywords : oral cancer, squamous cell carcinoma, adipokines, leptin, adiponectin