Effect of Direct Electrical Current on Protoscoleces of Echinococcus Granulosus in vitro and on Immune Response of BALB/c Mice Against Infection with Secondary Hydatid Disease

Authors : Asmaa Abdelazeez ALI, Fouad SALIM

Pages : 90-102

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Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :The present study investigated the effect of the direct electrical current on the viability of the protoscoleces of Echinococcus granulosus in vitro using different voltages of electrical current 3, 6, 9, 12 and 15, for different durations 3, 6, 9, 12 and 15 minutes, and on the immune response in BALB/c mice against infection with secondary hydatid cysts by injecting the mice with protoscoleces treated with direct electrical currents with viability 75%, 50%, 25% and 0%, in comparison with the control group, through six months, depending on many criteria included numbers, weights and diameters of the hydatid cysts and the percentage of reduction of their numbers, non-specific immune response represented by changes in the phagocytic index and specific immune response represented by delayed type hypersensitivity test insert ignore into journalissuearticles values(DTHT);.The results revealed that the effect of direct electrical current on protoscoleces viability in vitro increased with the increase in voltage and exposure time. the reduction in the numbers of hydatid cysts in treated mice was 100% , in groups injected with treated protoscoleces with viability 75%, 50% and 25%, respectively. A significant increase insert ignore into journalissuearticles values(p<0.01); in innate and cellular immune response in treated mice , represented by elevation in the rates of phagocytic index and delayed type hypersensitivity insert ignore into journalissuearticles values(foot pad thickness);.
Keywords : Direct electrical current, Echinococcus granulosus, Hydatid disease, Cell mediated immunity